Effects of human γ interferon on cell growth, replication of virus and induction of 2′-5′oligoadenylate synthetase in three human lymphoblastoid cell lines and K562 cells

Abstract

Human α interferon (IFN-α) and β interferon (IFN-β) showed antiviral and anticellular effects on human lymphoblastoid Daudi and P3HR-1 cells, but up to 1,000 units/ ml of γ interferon (IFN-γ) showed no such effect. Though a fairly high level of dsRNA-dependent 2′-5′-oligoadenylate synthetase (2–5A synthetase) was found in Daudi cells, treatment of these cells with IFN-α and β enhanced the enzyme level in cells at least four-fold, but IFN-γ did not show any such effect. Lymphoblastoid Raji cells were insensitive to the antiviral and anticellular activities of IFN-α, β and γ, but 2-5A synthetase was induced in cells by the treatment with IFN-α and β, though the enzyme level was lower than that found in interferon-treated Daudi cells. Human leukemic K562 cells were completely insensitive to IFN-α, β and at the same time to IFN-γ with regard to the antiviral, anticellular activities and to the induction of 2-5A synthetase.