Membrane currents in the rabbit atrioventricular node cell
- 1 March 1982
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 393 (1), 15-22
- https://doi.org/10.1007/bf00582385
Abstract
The rabbit A-V node was dissected into pieces (0.2×0.2×0.2 mm) smaller than its space constant of 692±96 μm (n=5). These small specimens showed spontaneous action potentials whose configurations were similar to those of large specimens before dissection. The membrane time constant was 21.5±1.5 ms (n=5). Voltage clamp experiments were performed on the above specimens using the two-microelectrode technique. On depolarization from the holding potential of −40 mV to various potential levels a transient inward current and delayed outward current were recorded. On repolarization an outward current tail was observed. The transient inward current was blocked by application of D600 (2×10−7 g/ml) but was insensitive to TTX (1×10−7 g/ml). The inward current was decreased by superfusion with Na- or Ca-free Tyrode solution. Thus, this current was classified as the slow inward current (i s ). When the K concentration in the Tyrode solution was varied, the reversal potential of the outward current tail changed as expected for a K electrode, indicating that the outward current was carried by K ions. On hyperpolarization slow activation of inward current was recorded. The reversal potential of this current was between −20 and −30 mV, which was analogous to hyperpolarization activated current,i h , in the S-A node. A contribution of sodium current (i Na ) to the action potential was obviously demonstrated from an inhibitory effect of TTX on the upstroke of the anodal break excitation. The ionic selectivity of each current system is compared with analogous current systems in other cardiac tissues and a possible mechanism for the slow conduction in the A-V node is discussed.This publication has 39 references indexed in Scilit:
- Slow current systems in the A-V node of the rabbit heartNature, 1980
- The Spontaneous Action Potential of Rabbit Atrioventricular Node CellsThe Japanese Journal of Physiology, 1980
- How does adrenaline accelerate the heart?Nature, 1979
- Membrane currents in the rabbit sinoatrial node cell as studied by the double microelectrode methodPflügers Archiv - European Journal of Physiology, 1976
- Effect of Verapamil on the Sinoatrial and Atrioventricular Nodes of the Rabbit and the Mechanism by Which it Arrests Reentrant Atrioventricular Nodal TachycardiaCirculation Research, 1974
- THE EFFECT OF POLARIZATION ON THE ACTION POTENTIALS OF THE RABBIT AV NODAL CELLSThe Japanese Journal of Physiology, 1974
- A STUDY OF THE MEMBRANE CONSTANTS IN THE DOG MYOCARDIUMThe Japanese Journal of Physiology, 1970
- Junctional Membrane UncouplingThe Journal of general physiology, 1967
- ELECTROPHYSIOLOGICAL PROPERTIES OF THE CANINE VENTRICULAR FIBERThe Japanese Journal of Physiology, 1966
- Tetrodotoxin Blockage of Sodium Conductance Increase in Lobster Giant AxonsThe Journal of general physiology, 1964