A Method for the Determination of Formiminoglutamic Acid in Urine1
Open Access
- 1 June 1958
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 37 (6), 824-828
- https://doi.org/10.1172/jci103670
Abstract
A sensitive spectrophotometric method is presented for the assay of formiminoglutamic acid in urine. This is based on the enzymatic reactions: (1) formiminoglutamic acid + tetrahydrofolic acid 5-formiminotetrahydrofolic acid + glutamic acid; (2) 5-formiminotetrahydrofolic acid [forward arrow]5,10-methenyl-tetrahydrofolic acid + NH3. Quantitative determination of 5,10-methenyl-tetrahydrofolic acid is carried out spectrophotometrically.This publication has 11 references indexed in Scilit:
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