Fluorescent differential display: Arbitrarily primed RT‐PCR fingerprinting on an automated DNA sequencer
- 5 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 351 (2), 231-236
- https://doi.org/10.1016/0014-5793(94)00867-1
Abstract
We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.Keywords
This publication has 14 references indexed in Scilit:
- Stress-inducible gene of Salmonella typhimurium identified by arbitrarily primed PCR of RNA.Proceedings of the National Academy of Sciences, 1994
- RNA fingerprinting using arbitrarily primed PCR identifies differentially regulated RNAs in mink lung (Mv1Lu) cells growth arrested by transforming growth factor beta 1.Proceedings of the National Academy of Sciences, 1993
- The Short 3′-End Region of Complementary DNAs as PCR-Based Polymorphic Markers for an Expression Map of the Mouse GenomeGenomics, 1993
- Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimizationNucleic Acids Research, 1993
- [24] Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: Thermal raceMethods in Enzymology, 1993
- Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain ReactionScience, 1992
- Arbitrarily primed PCR fingerprinting of RNANucleic Acids Research, 1992
- DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research, 1990
- Fingerprinting genomes using PCR with arbitrary primersNucleic Acids Research, 1990
- Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reactionGenomics, 1989