Composition of the Aliphatic Components of Suberin of the Endodermal Fraction from the First Internode of Etiolated Sorghum Seedlings

Abstract

The stems from etiolated seedlings of S. bicolor were separated into epidermal and endodermal fractions by manually removing the stele from the cortex. The epidermal fraction contained a lipid polymer whose monomeric composition was characteristic of cutin with dihydroxyhexadecanoic acid as a major component (25%). The endodermal fraction contained a lipid polymer whose monomeric composition was characteristic of suberin: hexadecanoic acid 12%, octadecenol 6%, octadecenoic acid 23%, .omega.-hydroxyhexadecanoic acid 17%, hexadecanedioc acid 8%, .omega.-hydroxyoctadecenoic acid 8% and octadecenedioic acid 12%. This endodermal polymer is thought to be the suberin component of the Casparian band.