Amino acid sequence in mycobacillin

Abstract

Mycobacillin was partially hydrolyzed and the hydrolysate fractionated by 2-dimensional paper chromatography to give a separation of complex mixtures of peptides. Altogether 15 peptides, including 7 dipeptides, 6 tripeptides and 2 tetrapeptides, were isolated. The N-terminal residue of each peptide was determined by both the deamination method and the dinitro-phenylation method. As the mycobacillin molecule does not contain a N-terminal residue, a cyclic structure has been suggested.