Uracil in formic acid hydrolysates of deoxyribonucleic acid
- 31 December 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 98 (1), 311-316
- https://doi.org/10.1042/bj0980311
Abstract
When DNA is hydrolysed with formic acid for 30 min. at 175[degree] and the hydrolysate is chromatographed on paper with propan-2-ol-2N-hydrochloric acid, in addition to expected ultraviolet-absorbing spots corresponding to guanine, adenine, cytosine and thymine, an ultraviolet-absorbing region with RF similar to that of uracil can be detected. Uracil was separated from this region and identified by its spectra in acid and alkali, and by its RF in several solvent systems. Cytosine, deoxyribocytidine and deoxyribocytidylic acid similarly treated with formic acid all yielded uracil, as did a mixture of deoxyribonucleotides. Approx. 4% of deoxyribonucleotide cytosine was converted into uracil by the formic acid treatment.This publication has 13 references indexed in Scilit:
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