Fractionation and specificities of two aspartyl-ribonucleic acid and two phenylalanyl-ribonucleic acid synthetases.
- 1 January 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 55 (1), 184-189
- https://doi.org/10.1073/pnas.55.1.184
Abstract
Using hydroxyapatite chromatography and transfer countercurrent distribution, it was observed that Neurospora contains 2 phenylalanyl-RNA synthetases and 2 phenylalanine-specific tRNA''s. The 2 phenylalanine tRNA''s are aminoacylated by different synthetases. Two aspartyl-RNA synthetases have also been separated, using diethylaminoethyl cellulose chromatography. These 2 enzymes differ in their specificity for the species of aspartic acid tRNA. The heat stabilities of the 2 aspartyl-RNA synthetases are very different. Neurospora contains synthetase(s) and transfer RNA(s) for L-asparagine which are distinct from those for L-aspartic acid.Keywords
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