Inhibition of DNA synthesis by cross-linking the template to platinum-thiol derivatives of complementary oligodeoxynucleotides
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (12), 4783-4798
- https://doi.org/10.1093/nar/17.12.4783
Abstract
Unsubstituted oligodeoxynucleotides, or oligodeoxynucleotides linked to poly-(L)-lysine, when hybridized to a 322 base long template, did not inhibit the production of full length DNA copies by the Klenow fragment of Escherichia coli DNA polymerase I. However, synthesis was inhibited if the cysteamine derivative of the same oligomer was cross-linked to the template via PtII. Truncated products were formed by termination of DNA synthesis a small number of bases upstream from the 5''-end of the cross-linked oligomer. AMV reverse transcriptase behaved similarly but was also slightly inhibited by the hybridized oligomer or its poly-lysine derivative.Keywords
This publication has 25 references indexed in Scilit:
- Photochemical cross-linking of psoralen-derivatized oligonucleoside methylphosphonates to rabbit globin messenger RNABiochemistry, 1988
- Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.Proceedings of the National Academy of Sciences, 1988
- Role of RNase H in hybrid-arrested translation by antisense oligonucleotides.Proceedings of the National Academy of Sciences, 1988
- Interaction of psoralen-derivatized oligodeoxyribonucleoside methylphosphonates with single-stranded DNABiochemistry, 1988
- Sequence-specific binding and photocrosslinking of alpha and beta oligodeoxynucleotides to the major groove of DNA via triple-helix formation.Proceedings of the National Academy of Sciences, 1988
- Ligation of oligonucleotides to nucleic acids or proteins via disulfide bondsNucleic Acids Research, 1988
- Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.Proceedings of the National Academy of Sciences, 1987
- Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.Proceedings of the National Academy of Sciences, 1987
- [Epidemiological analysis of the influenza virus A carrier state in healthy children].1983
- Catalysis of accurate poly(C)-directed synthesis of 3′-5′-linked oligoguanylates by Zn2+Journal of Molecular Biology, 1980