The isolation of a hypertensin

Abstract

Hypertensin formed by the action of rabbit renin on ox serum was resolved by partition chromatography into 3 active components. The amphoteric nature of the major active component was shown by electrophoresis on paper. The major active component was isolated in a form which appears to be homogeneous It is a peptide containing 8 different amino acids which are present as 10 residues in its mimimum stoichiometric unit. It shows a pressor action up to 3 times that of (-) nor-epinephrine. The hypertensin isolated in this work was compared with hypertensins previously described.