Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro
- 19 May 1994
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 369 (6477), 207-212
- https://doi.org/10.1038/369207a0
Abstract
Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-α/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-δ to replicate both strands of the double helix conjointly.Keywords
This publication has 51 references indexed in Scilit:
- SV40 DNA ReplicationPublished by Springer Nature ,1992
- Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase alpha-primase.Molecular and Cellular Biology, 1991
- Binding and unwinding—How T antigen engages the SV40 origin of DNA replicationCell, 1990
- Initiation of Eukaryotic DNA Replication In VitroAnnual Review of Cell Biology, 1989
- ANIMAL VIRUS DNA REPLICATIONAnnual Review of Biochemistry, 1989
- p53 and DNA polymerase α compete for binding to SV40 T antigenNature, 1987
- In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.Proceedings of the National Academy of Sciences, 1985
- Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.Molecular and Cellular Biology, 1985
- Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication.Molecular and Cellular Biology, 1985
- Simian virus 40 DNA replication in vitro.Proceedings of the National Academy of Sciences, 1984