ON THE SOURCES OF CARBON FOR THE INDUCED BIOSYNTHESIS OF ALPHA-AMYLASE IN PSEUDOMONAS SACCHAROPHILA

Abstract
In a growth experiment with isotopically labeled cells a small but significant portion of the alpha-amylase was synthesized from pre-existing C compounds of the cell. With C14-labeled resting cells about 65% of the C for the induced biosynthesis of alpha-amylase come from C compounds of the cell. Using C14-labeled starch as substrate for resting cells, approximately 39% of the enzyme C comes from the substrate. A free amino acid and peptide pool was demonstrated in P. saccharophila and some data on its composition given.