Cyclic AMP Induction of the Myelin Enzyme 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Rat Oligodendrocytes
- 1 February 1983
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (2), 506-515
- https://doi.org/10.1111/j.1471-4159.1983.tb04768.x
Abstract
CAMP is known to induce the activity of the myelin enzyme 2'',3''-cyclic nucleotide 3''-phosphohydrolase (CNP; EC 3.1.4.37) in C6 rat glioma cells. CNP is also inducible in oligodendrocytes explanted from 1-day-old rat cerebrum and grown in tissue culture. Induction was observed after a 1-day treatment with 1 mM N6, O2''-dibutyryl cAMP (dbcAMP) and was maximal after 5 days, reaching 200-240% of control. Induction was observed both in mixed cerebral cell cultures containing oligodendrocytes and astrocytes, and in purified cultures of oligodendrocytes prepared by a differential shakeoff procedure. Addition of dbcAMP to the cultures 3-9 days after the cells were explanted from rat brain induced CNP activity, but no induction was observed when dbcAMP treatment was begun 13 or more days after explantation. One component of myelin, CNP, is inducible in oligodendrocytes by a cAMP-mediated mechanism, and suggest a role for cAMP in the regulation of the myelin-associated functions of oligodendrocytes.Keywords
This publication has 29 references indexed in Scilit:
- Demonstration of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Cultured Human Schwann CellsJournal of Neurochemistry, 1982
- The Activity of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in Rat TissuesJournal of Neurochemistry, 1982
- Evidence for the Association of 2′,3′‐Cyclic‐Nucleotide 3′‐Phosphodiesterase with Myelin‐Related Membranes in Peripheral Nervous SystemJournal of Neurochemistry, 1980
- Comparison of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase and the Major Component of Wolfgram Protein W1Journal of Neurochemistry, 1980
- Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.The Journal of cell biology, 1980
- ACTIVITY OF 2′,3′‐CYCLIC‐NUCLEOTIDE 3′‐PHOSPHODIESTERASE IN REGIONS OF RAT BRAIN DURING DEVELOPMENT: QUANTITATIVE RELATIONSHIP TO MYELIN BASIC PROTEINJournal of Neurochemistry, 1978
- Surface culture of fetal mammalian brain cells: Effect of subculture on morphology and choline acetyltransferase activityJournal of Neurobiology, 1973
- ISOLATION AND SOME CHEMICAL PROPERTIES OF OLIGODENDROGLIA FROM CALF BRAIN1Journal of Neurochemistry, 1972
- 2′,3′-cyclic nucleotide 3′-phosphohydrolase in human erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- THE REGIONAL AND SUBCELLULAR DISTRIBUTION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE IN THE CENTRAL NERVOUS SYSTEMJournal of Neurochemistry, 1967