Ag-staining of nucleolus organizer regions of chromosomes after Q-, C-, G-, or R-banding procedures

Abstract

Metaphase chromosome preparations were made from leukocyte cultures of normal individuals. Quinacrine staining followed by Ag-staining resulted in well-defined, darkly stained NORs [nucleolus organizer regions]. Similar results were obtained if the slides were stained with quinacrine, restained to reveal C[centromeric heterochromatin]-bands, and finally Ag-stained to reveal the NORs. This combination appears to offer the opportunity to study the maximum number of chromosomal markers because different polymorphic regions are revealed by each of the 3 methods.