Targeting of a chimeric human histone fusion mRNA to membrane-bound polysomes in HeLa cells.
- 1 May 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (9), 2683-2687
- https://doi.org/10.1073/pnas.84.9.2683
Abstract
The subcellular location of histone mRNA-containing polysomes may play akey role the posttranscriptional events that mediate histone mRNA turnover following inhibition of DNA synthesis. Previously, it has been shown that histone mRNA is found primarily on free polysomes that are associated with the cytoskeleton. We report here the construction of an Escherichia coli pBR322 .beta.-lactamase signal peptide-human H3 histone fusion gene. The fusion transcript is targeted to membrane-bound polysomes and remains stable following interruption of DNA replication. Relocating mRNA within the cell may provide a procedure for studying the posttranscriptional regulation of gene expression.Keywords
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