Association of the fyn protein-tyrosine kinase with the T-cell antigen receptor.
- 1 June 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (11), 4358-4362
- https://doi.org/10.1073/pnas.87.11.4358
Abstract
Activation of the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of the TCR .delta. chain and other intracellular substrates. Two other T-cell integral membrane proteins, CD4 and CD8, are associated with the protein-tyrosine kinase (PTK), lck. Despite evidence that activation of this enzyme results in TCR-.delta. chain phosphorylation, it has not been shown that the TCR activates lck. We have sought evidence that the TCR is associated with a PTK. In this study we use digitonin to solubilize a murine T-cell hybridoma and demonstrate that antibodies binding extracellular but not intracellular domains of the TCR specifically coprecipitate only the fyn PTK and not lck or yes, two other kinases found in these cells. The association of the fyn PTK with the TCR might enable the T cell to independently regulate two PTKs through surface receptors.This publication has 24 references indexed in Scilit:
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