Multivariate Methods to Evaluate the Role of Mixed Supports in Reversed-Phase Thin-Layer Chromatography

Abstract

Hydrophobic properties of 17 aniline and phenol derivatives were characterized by reversed-phase thin-layer chromatographic and high performance liquid chromatographic retention data. In order to elucidate the role of thin-layer chromatographic supports in the hydrophobicity determination paraffin coated silica, aluminium oxide, cellulose, diatomaceous earth and their mixtures were used. Water, water-methanol 7:3 and 1 M NaCl served as mobile phases. The retention data were analyzed by spectral mapping technique. The potency values differed from support to support proving that the composition of support has a deciding role in the hydrophobicity determination of aniline and phenol derivatives. The eluents did not influence considerably the potency order of supports.