Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction
- 1 January 1990
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 1 (3), 228-232
- https://doi.org/10.1002/gcc.2870010307
Abstract
We have studied 31 male germ cell tumors (GCTs) for probable mutations in codons 12, 13, and 61 of HRAS, KRAS, and NRAS oncogenes using the polymerase chain reaction. Twenty of the thirty-one tumors exhibited NRAS gene mutations, 14 in codon 61, and six in codon 12, whereas no mutations were detected in HRAS and KRAS genes. The NRAS mutations were equally prevalent in seminomatous and nonseminomatous GCTs. Thus 13 of 22 seminomas, six of seven embryonal carcinomas, and one of two mixed tumors exhibited mutations. Two non-seminomatous tumors (an embryonal carcinoma and a yolk sac/teratoma) had mutations in both codons 12 and 61. The high frequency of NRAS mutations observed in the present study suggests that NRAS gene products may play an important role in growth regulatory functions of premalignant and malignant germ cells.This publication has 14 references indexed in Scilit:
- Mutations in the KRAS2 oncogene during progressive stages of human colon carcinoma.Proceedings of the National Academy of Sciences, 1989
- Genetic Alterations during Colorectal-Tumor DevelopmentNew England Journal of Medicine, 1988
- A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotidesGene, 1986
- Chromosome changes in germ cell tumors of the testisCancer Genetics and Cytogenetics, 1986
- Chromosomal localization of three humanras genes by in situ molecular hybridizationSomatic Cell and Molecular Genetics, 1985
- Homologies Between Signal Transducing G Proteins and ras Gene ProductsScience, 1984
- The product of ras is a GTPase and the T24 oncogenic mutant is deficient in this activityNature, 1984
- SPECIFIC CHROMOSOME CHANGE, i(12p), IN TESTICULAR TUMOURS?The Lancet, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Simple and rapid fluorimetric method for DNA microassayAnalytical Biochemistry, 1977