High level synthesis of immunoglobulins in Chinese hamster ovary cells.
- 1 November 1990
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 145 (9), 3011-3016
- https://doi.org/10.4049/jimmunol.145.9.3011
Abstract
The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.This publication has 26 references indexed in Scilit:
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