Multiple steps in DNA recognition by restriction endonuclease from E. coli K
- 14 August 1975
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 256 (5518), 556-560
- https://doi.org/10.1038/256556a0
Abstract
The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme–DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.Keywords
This publication has 11 references indexed in Scilit:
- Cleavage map of bacteriophage f1: Location of the Escherichia coli B-specific modification sitesJournal of Molecular Biology, 1975
- Restriction of bacteriophage λ by Escherichia coli KJournal of Molecular Biology, 1973
- DNA targets for the Escherichia coli K restriction system analysed genetically in recombinants between phages Phi80 and lambdaMolecular Genetics and Genomics, 1973
- ATP Hydrolysis by Restriction Endonuclease from E. coli KNature New Biology, 1972
- Cleavage of Bacteriophage f1 DNA by the Restriction Enzyme of Escherichia coli BProceedings of the National Academy of Sciences, 1972
- DNA Modification Methylase Activity of Escherichia coli Restriction Endonucleases K and PProceedings of the National Academy of Sciences, 1972
- Host specificity of DNA produced by Escherichia coli: XIV. the role of nucleotide methylation in in vivo B-specific modificationJournal of Molecular Biology, 1972
- Host specificity of DNA produced by Escherichia coli: XV. The role of nucleotide methylation in in vitro B-specific modificationJournal of Molecular Biology, 1972
- A Specific Complex between a Restriction Endonuclease and Its DNA SubstrateProceedings of the National Academy of Sciences, 1970
- DNA Restriction Enzyme from E. coliNature, 1968