Interaction of Compound 48/80 and Stilbamidine with Strandin.

Abstract

The histamine releasers, compound 48/80 and stilbamidine interact directly with the brain ganglioside, strandin, to form turbidimetric products at 6[degree]C. The fluorescence of stilbamidine is quenched by strandin, the effect being a function of the strandin concentration. Strandin exerts a marked effect on the UV spectrum of stilbamidine. Compound 48/80 causes a reduction in the turbidity of the strandin-lysozyme and the strandin-protamine systems. In the case of lysozyme, the reaction was reversed by readdition of strandin. While similar results were obtained with stilbamidine and both lysozyme-strandin and poly-i-lysine-strandin, an increase in turbidity resulted when it was added to the protamine-strandin system. Strandin effectively suppressed certain polysulfonate-stilbamidine interactions. A reversible equilibrium between these compounds and strandin is proposed to explain the results obtained.