Irradiation of Solutions of Transforming DNA
- 1 July 1959
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 11 (1), 38-53
- https://doi.org/10.2307/3570732
Abstract
Transforming DNA has been irradiated in solution over a wide range of concentrations, and the ionic yield determined for inactivation of the biological transforming activity. The D37''s ranged from 65 r to several thousand roentgens, depending on the DNA preparation and the concentration. The maximum observed yield indicated that a mass of 500,000 molecular weight was inactivated per ion pair, which corresponds closely to the sensitivity observed for direct action. No aftereffect of irradiation was observed, nor was an effect of removing O2 found. In sufficiently pure solutions one may observe the effect of at least two intermediates, suspected as being OH and H. In vitro a radical has a probability near unity of inactivating a DNA molecule biologically if it gets to the molecule, but in vivo relatively few of the radicals produced do find the DNA.Keywords
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