A mass-spectrometric method for the estimation of the ratio of γ-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin
- 1 April 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 187 (1), 239-243
- https://doi.org/10.1042/bj1870239
Abstract
When a polypeptide containing .gamma.-carboxyglutamic acid is decarboxylated in 2H2O, residue of (.gamma..gamma.-2H2)glutamic acid are formed. Subsequent proteolytic digestion produces peptides which contain at each site 2H2-substituted and unsubstituted glutamic acid in the same ratio as existed for .gamma.-carboxy-substitution. The peptides may be identified and this ratio determined by combined gas chromatography-mass spectrometry. Decarboxylation in 3H2O followed by amino-acid analysis and Edman degradation are also discussed.This publication has 11 references indexed in Scilit:
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