Localization of the main immunogenic region of human muscle acetylcholine receptor to residues 67-76 of the alpha subunit.
- 1 May 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (9), 2899-2903
- https://doi.org/10.1073/pnas.85.9.2899
Abstract
The majority of antibodies to the acetylcholine receptor (AcChoR), both in the human disease myasthenia gravis and in its experimental models, are directed against an extracellular area of the AcChoR .alpha. subunit called the main immunogenic region (MIR). We have studied the binding of anti-AcChoR monoclonal antibodies (mAbs) to 26 synthetic peptides corresponding to the hydrophilic parts of the human AcChoR .alpha. subunit. The binding sites for eight anti-MIR mAbs and for eight anti-.alpha.-subunit, non-anti-MIR mAbs were localized. Anti-MIR mAbs bound to one peptide corresponding to residues 63-80 of the human .alpha. subunit. A second panel of peptides corresponding to the various parts of the .alpha.-subunit segment 63-80 was synthesized. Anti-MIR antibodies bound to a peptide that contained the .alpha.-subunit sequence 67-76. Thus, a main constituent loop of the MIR is localized between residues 67 and 76 of the .alpha. subunit.This publication has 37 references indexed in Scilit:
- Tests of the helix dipole model for stabilization of α-helicesNature, 1987
- Three-dimensional structure of a complex of antibody with influenza virus neuraminidaseNature, 1987
- The antibody response to myoglobin—I. Systematic synthesis of myoglobin peptides reveals location and substructure of species-dependent continuous antigenic determinantsMolecular Immunology, 1986
- Transmembrane topography of the nicotinic acetylcholine receptor: immunochemical tests contadict theoretical predictions based on hydrophobicity profilesBiochemistry, 1986
- Location of antigenic determinants of primary sequences of the subunits of nicotinic acetylcholine receptor by peptide mappingBiochemistry, 1986
- Myasthenia gravis and myasthenic syndromesAnnals of Neurology, 1984
- Demonstration of a main immunogenic region on acetylcholine receptors from human muscle using monoclonal antibodies to human receptorFEBS Letters, 1983
- Structural homology of Torpedo californica acetylcholine receptor subunitsNature, 1983
- Mammalian Muscle Acetylcholine Receptor: A Supramolecular Structure Formed by Four Related ProteinsScience, 1982
- Monoclonal antibodies as probes of acetylcholine receptor structure. 2. Binding to native receptorBiochemistry, 1981