Actinophage R4 as a DNA cloning vector in Streptomyces.
- 1 January 1981
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 27 (5), 373-379
- https://doi.org/10.2323/jgam.27.373
Abstract
Viable deletion mutants of actinophage R4 were isolated using a pyrophosphate treatment. One of the deletion mutants, named R4.DELTA.2, had a deletion of 0.98 megadaltons. A successful cloning of foreign DNA previously cleaved by SmaI endonuclease demonstrated that a unique PvuII site of R4.DELTA.2 DNA was located in a dispensable region. The usefulness of R4 deletion mutants as cloning vectors in Streptomyces is discussed.This publication has 9 references indexed in Scilit:
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