The steady state, a test for electrophoretic homogeneity

Abstract

The theoretical shape of the pattern of a boundary in the steady state has been calculated. A steady state, indicating electrophoretic homogeneity, has been realized experimentally with the HbO2 of normal human red-cell extracts. With crystallized bovine albumin, or with the albumin in a serum from a patient with hypertension, no steady state was attained. These materials were considered to be heterogeneous, although they showed single peak during prolonged electro-phoresis. A method of testing for electrophoretic homogeneity has been outlined. The presence in red-cell extracts of two electrophoretic components, not hitherto described, has been demonstrated.