Promiscuity of GABAA‐receptor β3 subunits as demonstrated by their presence in α1, α2 and α3 subunit‐containing receptor subpopulations
- 16 December 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 295 (1-3), 81-83
- https://doi.org/10.1016/0014-5793(91)81390-t
Abstract
Polyclonal antibodies were raised in rabbits against the GABAA-receptor β3 subunit peptide sequence, KQSMPREGHGRHMDR-NH2 coupled to keyhole limpet haemocyanin. These anti-β3 379–393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na+ deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-β3 379–393 antibodies recognised two species with Mfr 59 900 and Mfr 57 200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-α1 324–341 antibody, anti-Cys α2 414–424 antibody and anti-Cys α3 454–467 antibody immunoaffinity-purified GABAA-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABAA-receptor β3 subunitKeywords
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