Uncoupling reagents and metabolism. 1. Effects of salicylate and 2:4-dinitrophenol on the incorporation of 14C from labelled glucose and acetate into the soluble intermediates of isolated rat tissues

Abstract

The effects of 5 m[image]-sali-cylate and 0.5 m[image]-2,4-dinitrophenol on the incorporation of C14 from [C14] glucose and [2-C14] acetate into the soluble intermediates of chopped rat tissues has been studied. In the absence of the uncoupling reagents, the tissue preparations incorporated the isotope into substances known to be involved in glucolysis, the hexose monophosphate oxidative pathway, the tricarboxylic acid cycle, transamination and synthetic reactions. Salicylate and dinitrophenol considerably reduced the total incorporation of C14 from the labelled acetate, and the subsequent distribution of the isotope was restricted to the amino and organic acid fractions of the soluble intermediates. The total incorporation and distribution of the C14 from the [C14] glucose were not materially altered in the presence of salicylate and dinitrophenol. Some implications of these results are discussed.