Identification of fusobacteria in a routine diagnostic laboratory
- 1 August 1985
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 59 (2), 171-181
- https://doi.org/10.1111/j.1365-2672.1985.tb03318.x
Abstract
A scheme for differentiating Fusobacterium spp. and Leptotrichia spp. from Bacteroides spp. was derised after examining 114 strains of fusobacteria and asacharolytic bacteroides (17 reference strains and 97 clinical isolates). Sensitivity to a 300 .mu.g/ml plate of phosphomycin and an acid reaction on a lysine plate were found to be reliable for differentiating Fusobacterium spp. and L. buccalis from Bacteroides. Using a short set of simple cultural and biochemical tests, isolates could be identified as F. necrophorum, F. necrogenes, F. nucleatum, F. varium or L. buccalis. These tests were: indole, lecithinase, phosphatase, DNase and gas production, esculin and casein hydrolysis, greening of casein/methylene blue agar, nitrite reduction, bile tolerance and hemolysis on horse blood agar.This publication has 13 references indexed in Scilit:
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