A note on the chromatographic identification of blood keto acids in animals poisoned with arsenite and alloxan

Abstract

A chromatographic study was made of the keto acids present in the blood of normal rats and of rats poisoned with sodium arsenite and alloxan, respectively. It was shown that a rise in the blood pyruyate level is accompanied by a rise in the "third spot" visible in chromatograms of dinitrophenyl hydrazones from blood, and that no increase in this occurs when the acetoacetate level is high. It is concluded that this third spot is due to the more rapidly running component of the pyruvic acid 2:4-dinitrophenylhydrazone, and that high acetoacetate levels are not likely to interfere in the chromatographic estimation of the blood pyruvate level.