Single Step Purification of Locust Adipokinetic Hormones I and II by Reversed-Phase High-Performance Liquid Chromatography, and Amino-Acid Composition of the Hormone II
- 1 January 1984
- journal article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (1), 393-398
- https://doi.org/10.1515/bchm2.1984.365.1.393
Abstract
Locust adipokinetic hormones I (AKH I) and II (AKH II) are separated by reversed-phase high-performance liquid chromatography using a mu-Bondapak phenyl column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hyperlipaemic activity was detected using a bioassay. The amino-acid composition of AKH II was determined after acid hydrolysis with HCl or methane-sulfonic acid. It contained the following amino-acid residues in almost equimolar amounts: Asp, Ser, Glu, Gly, Ala, Leu, Phe and Trp.Keywords
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