ISOLATION AND CHARACTERIZATION OF RAT PANCREATIC SOMATOSTATIN
- 1 December 1980
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 107 (6), 2127-2129
- https://doi.org/10.1210/endo-107-6-2127
Abstract
A peptide representing the major form of somatostatin-like immunoreactivity was isolated from 600 rat pancreata by using anti-somatostatin affinity chromatography, gel permeation chromatography and reverse-phase high-performance liquid chromatography (HPLC). The isolated peptide elutes with the same retention time as synthetic somatostatin-14 in isocratic HPLC and its amino acid composition is in agreement with that of the tetradecapeptide. We propose that the structure of the major rat pancreatic somatostatin is identical to that of somatostatin-14 characterized in other species.Keywords
This publication has 6 references indexed in Scilit:
- Solid phase synthesis of somatostatin-28Biochemical and Biophysical Research Communications, 1980
- Identification of prosomatostatin in pancreatic islets.Proceedings of the National Academy of Sciences, 1980
- Isolation and Characterization of Somatostatin from Anglerfish Pancreatic Islet*Endocrinology, 1979
- Isolation and characterization of somatostatin from pigeon pancreas.Proceedings of the National Academy of Sciences, 1979
- Subcellular distribution of radioimmunoassayable somatostatin in rat brainBrain Research, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976