Effect of the Defoliant Thidiazuron on Ethylene Evolution from Mung Bean Hypocotyl Segments
- 1 August 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 75 (4), 902-907
- https://doi.org/10.1104/pp.75.4.902
Abstract
The effect of the defoliant thidiazuron (N-phenyl-N''1,2,3-thiadiazol-5-ylurea) on ethylene evolution from etiolated mung bean hypocotyl segments was examined. Treatment of hypocotyl segments with concentrations of thidiazuron .gtoreq. 30 nM stimulated ethylene evolution. Increased rates of ethylene evolution from thidiazuron-treated tissues could be detected within 90 min of treatment and persisted up to 30 h after treatment. Radioactive [14C] methionine was readily taken up by thidiazuron-treated tissues and was converted to ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC) and an acidic conjugate of ACC. Aminoethoxyvinylglycine, aminooxyacetic acid, cobalt chloride and .alpha.-aminoisobutyric acid reduced ethylene evolution from treated tissues. An increase in the endogenous content of free ACC coincided with the increase in ethylene evolution following thidiazuron treatment. Uptake and conversion of exogenous ACC to ethylene were not affected by thidiazuron treatment. No increases in the extractable activities of ACC synthase were detected following thidiazuron treatment.This publication has 5 references indexed in Scilit:
- Identification and Metabolism of 1-(Malonylamino)cyclopropane-1-carboxylic Acid in Germinating Peanut SeedsPlant Physiology, 1983
- Identification of 1-(malonylamino)cyclopropane-1-carboxylic acid as a major conjugate of 1-aminocyclopropane-1-carboxylic acid, an ethylene precursor in higher plantsBiochemical and Biophysical Research Communications, 1982
- A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acidAnalytical Biochemistry, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco LeavesPlant Physiology, 1976