Use of Drug-level Testing and Single-genome Sequencing to Unravel a Case of Human Immunodeficiency Virus Seroconversion on Pre-exposure Prophylaxis

Abstract
Cases of seroconversion on pre-exposure prophylaxis (PrEP) should be carefully investigated, given their public health implications and rarity. We report a case of transmitted drug resistance causing seroconversion on PrEP in spite of high adherence, confirmed with dried blood spot and segmental hair drug-level testing and single-genome sequencing.
Funding Information
  • US Department of Health and Human Services
  • National Cancer Institute (K23MH12286)
  • National Institute of Mental Health (K23MH12286)
  • National Institutes of Health (K23MH12286)
  • National Institute of Allergy and Infectious Diseases (R01AI118575, R01AI122298, R01AI143340, 2R01AI098472)
  • U.S. Department of Health and Human Services

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