Use of Hoechst Dyes 33258 and 33342 for Enumeration of Attached and Planktonic Bacteria
- 1 April 1982
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 43 (4), 939-944
- https://doi.org/10.1128/aem.43.4.939-944.1982
Abstract
The DNA-specific fluorochromes Hoechst 33258 and 33342 were used to enumerate aquatic bacteria by epifluorescent direct counts. Cultures of estuarine bacteria gave identical counts when stained with Hoechst 33258 or acridine orange, whereas natural populations of aquatic bacteria gave 92-98.5% of the acridine orange counts. The technique had distinct advantages over acridine orange when enumerating bacteria on surfaces which bind acridine orange, such as polystyrene.This publication has 15 references indexed in Scilit:
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