Isolation and characterization of cell hybrids containing human Xp-chromosome fragments
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 54 (1-2), 10-14
- https://doi.org/10.1159/000132945
Abstract
We have subjected C12D, a Chinese hamster hybrid containing only the human X chromosome, to 6-thioguanine selection. The majority of the derivative clones retained rearranged Xp-fragments, which were characterized by using a combination of enzyme markers, DNA probes, and in situ hybridization. Two of these, TG2 and TG5sc9.1, contained only an Xpter→p21 fragment and should be an ideal resource for directed cloning from this region. A possible mechanism for the specific retention of Xp-fragments is discussed.Keywords
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