Adverse effects of phenobarbital on morphological and biochemical development of fetal mouse spinal cord neurons in culture
- 1 June 1981
- journal article
- research article
- Published by Wiley in Annals of Neurology
- Vol. 9 (6), 584-589
- https://doi.org/10.1002/ana.410090612
Abstract
The effects of chronic phenobarbital treatment on neuronal development were assessed in cell cultures of fetal rodent spinal cord. The activity of the principal enzyme involved in acetylcholine synthesis, choline acetyltransferase (CAT), and counts of large spinal cord neurons were used as indicators of neuronal development. Phenobarbital (30 to 120 m̈g/ml) added to cultures for two- or six-week periods produced dose-dependent decreases in both CAT activity and neuron counts. Barbituric acid at doses equimolar to those at which phenobarbital produced these decreases had no effect.Keywords
This publication has 16 references indexed in Scilit:
- Neuronal maturation in mammalian cell culture is dependent on spontaneous electrical activityBrain Research, 1981
- Neuronal deficits in mice following prenatal exposure to phenobarbitalExperimental Neurology, 1979
- Decreased brain weights in rats after long-term barbital treatmentsLife Sciences, 1978
- Choline acetyltransferase activity of spinal cord cell cultures increased by co-culture with muscle and by muscle-conditioned mediumThe Journal of cell biology, 1977
- Preferential neurotoxicity of pentobarbital on nerve and glial cells in cultureCellular and Molecular Life Sciences, 1977
- Phenobarbital-Induced Brain Growth Retardation in Artificially Reared Rat PupsNeonatology, 1977
- Neurons from fetal rat brain in a new cell culture system: A multidisciplinary analysisBrain Research, 1975
- Quantitative growth and development of human brainArchives of Disease in Childhood, 1973
- The effects of anticonvulsants onin vitro protein synthesis in immature brainBrain Research, 1973
- A SIMPLIFIED RADIOCHEMICAL ASSAY FOR CHOLINE ACETYLTRANSFERASEJournal of Neurochemistry, 1967