Structural Characterization of U*-1915 in Domain IV from Escherichia Coli 23S Ribosomal RNA as 3-Methylpseudouridine
- 1 February 1996
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (4), 688-693
- https://doi.org/10.1093/nar/24.4.688
Abstract
Mass spectrometry-based methods have been used to study post-transcriptional modification in the 1900-1974 nt segment of domain IV in 23S rRNA of Escherichia coli, a region which interacts with domain V in forming the three- dimensional structure of the peptidyl transferase center within the ribosome. A nucleoside constituent of M r 258 (U*)which occurs at position 1915, within the highly modified oligonucleotide sequence 1911-psiAACU*Apsi-1917, was characterized as 3-methylpseudouridine (m3psi). The assignment was confirmed by chemical synthesis of m3psi and comparison with the natural nucleoside by liquid chromatography-mass spectrometry. 3-Methylpseudouridine is previously unknown in nature and is the only known derivative of the common modified nucleoside pseudouridine thus far found in bacterial rRNA.Keywords
This publication has 39 references indexed in Scilit:
- A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosomeNature, 1995
- Pseudouridine and O2-methylated nucleosides. Significance of their selective occurrence in rRNA domains that function in ribosome-catalyzed synthesis of the peptide bonds in proteinsBiochimie, 1995
- Clustering of Pseudouridine Residues Around the Peptidyltransferase Center of Yeast Cytoplasmic and Mitochondrial RibosomesBiochemistry, 1994
- Four newly located pseudouridylate residues in Escherichia coli 23S ribosomal RNA are all at the peptidyltransferase center: Analysis by the application of a new sequencing techniqueBiochemistry, 1993
- Methylation sites in Escherichia coli ribosomal RNA: Localization and identification of four new sites of methylation in 23 S rRNABiochemistry, 1992
- Pseudouridine in the large-subunit (23 S-like) ribosomal RNA The site of peptidyl transfer in the ribosome?FEBS Letters, 1992
- Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNAJournal of Molecular Biology, 1986
- Studies on the primary structure of Escherichia coli 23S rRNA: III. Nucleotide sequence of the T1 RNase digestion products containing one or no uridine residueBiochimie, 1979
- Presence of a hypermodified nucleotide in hela cell 18 S and Saccharomyces carlsbergensis 17 S ribosomal RNAsFEBS Letters, 1975
- The methylated nucleotide sequences in HeLa cell ribosomal RNA and its precursorsJournal of Molecular Biology, 1974