A Silent Hemoglobin Variant Detected by Hplc: Hemoglobin City of Hope β69 (E13) Gly → Ser
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 8 (4), 333-342
- https://doi.org/10.3109/03630268408991716
Abstract
A silent hemoglobin variant with substitution of serine for glycine at position 69 of the 6-chain was discovered in a healthy individual. Reverse-phase HPLC was used for globin chain separation and to separate the tryptic peptides of the variant. This variant was undetectable by conventional methods of protein separation such as electrophoresis, iso-electric focusing, and ion-exchange chromatography. This observation demonstrates the potential of reverse-phase HPLC as a tool for the search and detection of neutral substitutions in variants of hemoglobin and other proteins, and its usefulness for screening genetic variations in human populations.This publication has 18 references indexed in Scilit:
- A Silent, Neutral Substitution Detected by Reverse-Phase High-Performance Liquid Chromatography: Hemoglobin BeirutScience, 1983
- Identification of eleven human hemoglobin variants by high-performance liquid chromatography: Additional data on functional properties and clinical expressionBiochemical Genetics, 1982
- Detection of Hb-Papio B, a Silent Mutation of the Baboon β Chain, by High Performance Liquid Chromatography. Improved Procedures for the Separation of Globin Chains by HplcHemoglobin, 1982
- Hemoglobin Great Lakes (beta 68 [E12] leucine replaced by histidine): a new high-affinity hemoglobinBlood, 1981
- Further Experiments in the Separation of Globin Chains by High Performance Liquid ChromatographyJournal of Liquid Chromatography, 1981
- Separation of tryptic peptides of normal and abnormal a, β, γ, and δ hemoglobin chains by high-performance liquid chromatographyJournal of Chromatography A, 1979
- Rapid separation of the α,β,Gγ and Aγ human globin chains by reversed-phase high pressure liquid chromatographyBiochemical and Biophysical Research Communications, 1979
- A Simple Method for the Detection of Unstable HaemoglobinsBritish Journal of Haematology, 1972
- The use of thermolysin in amino acid sequence determinationBiochemical Journal, 1968
- Abnormal human haemoglobins: Separation and characterization of the α and β chains by chromatography, and the determination of two new variants, Hb chesapeake and Hb J (Bangkok)Journal of Molecular Biology, 1966