Characterization of oligosaccharides from the urine of loco‐intoxicated sheep
- 29 October 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (1), 104-109
- https://doi.org/10.1016/0014-5793(83)81173-9
Abstract
Two major oligosaccharides were isolated by preparative HPLC from the urine of a locoweed-fed sheep. Analysis by gas-liquid chromatography and mass-spectrometry indicated compositions of (Man)4(GlcNAc)2 and (Man)5(GlcNAc)2, respectively. Structures were determined by digestion with α-D-mannosidase and endo-β-N-acetylglucosaminidases D and H, and comparison of the products by HPLC with synthetic standards, and oligosaccharides isolated from human mannosidosis urine. Incubation with an exo-β-N-acetylglucosaminidase was without effect.Keywords
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