Nanopore-Based Single-Molecule DNA Analysis
- 23 August 2007
- journal article
- review article
- Published by Taylor & Francis in Nanomedicine
- Vol. 2 (4), 459-481
- https://doi.org/10.2217/17435889.2.4.459
Abstract
Nanopore-based DNA analysis is a single-molecule technique with revolutionary potential. It promises to carry out a range of analyses, orders of magnitude faster than current methods, including length measurement, specific sequence detection, single-molecule dynamics and even de novo sequencing. The concept involves using an applied voltage to drive DNA molecules through a narrow pore that separates chambers of electrolyte solution. This voltage also drives a flow of electrolyte ions through the pore, measured as an electric current. When molecules pass through the pore, they block the flow of ions and, thus, their structure and length can be determined based on the degree and duration of the resulting current reductions. In this review, I explain the nanopore-based DNA analysis concept and briefly explore its historical foundations, before discussing and summarizing all experimental results reported to date. I conclude with a summary of the obstacles that must be overcome for it to realize its promised potential.Keywords
This publication has 113 references indexed in Scilit:
- Nanopore sequencing technology: research trends and applicationsTrends in Biotechnology, 2006
- Sequence-dependent gating of an ion channel by DNA hairpin moleculesNucleic Acids Research, 2006
- Cheminformatics methods for novel nanopore analysis of HIV DNA terminiBMC Bioinformatics, 2006
- DNA Molecule Classification Using Feature PrimitivesBMC Bioinformatics, 2006
- DNA counterion current and saturation examined by a MEMS-based solid state nanopore sensorBiomedical Microdevices, 2006
- Fast DNA Sequencing via Transverse Electronic TransportNano Letters, 2006
- Beyond the gene chipBell Labs Technical Journal, 2005
- Nanopore CheminformaticsDNA and Cell Biology, 2004
- Unzipping Kinetics of Double-Stranded DNA in a NanoporePhysical Review Letters, 2003
- Evaluation of nanopores as candidates for electronic analyte detectionElectrophoresis, 2002