Synthesis of 35S-labelled arylsulphates by intact animals and by tissue preparations, with particular reference to l-tyrosine O-sulphate

Abstract

Examination of normal human urine by column and paper chromatography confirmed the presence of L-tyrosine O-sulphate and revealed the presence of phenylsulphate, p-cresylsulphate and indican. Examination of the urine of rabbits, guinea pigs, rats and mice dosed with Na2S35O4 revealed the presence of s35-labelled phenylsulphate, p-cresylsulphate and indican, but not of L-tyrosine O-sulphate. By the use of a particle-free system prepared from rat liver and incubated in tris buffer, a brief study was made of the requirements of the sulphate-esterifying system which forms phenylsulphate from phenol. The arylsulphates of p-cresol, p-ethylphenol and p-acetylphenol were synthesized by this system. Liver- and kidney-tissue fractions of rabbits and guinea pigs synthesized phenylsulphate from phenol, as did a sample of fresh human liver. Tyramine underwent conjugation with sulphate during incubation with rat-liver-tissue preparation. This biosynthetic sulphate conjugate did not appear to be tyramine O-sulphate. No evidence was obtained for formation of L-tyrosine O-sulphate from L-tyrosine with any of the preparations used.