Bromobimane crosslinking studies in oligomycin‐sensitive ATPase from beef heart mitochondria
- 13 December 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 150 (1), 207-210
- https://doi.org/10.1016/0014-5793(82)81335-5
Abstract
Using a bromobimane fluorescent label the M r 31 000 protein band oligomycin‐sensitive (OS)‐ATPase from beef heart mitochondria is shown to become much intensified by 2‐mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP—Pi exchange activity is increased by 90%. With the fluorescent crosslinking reagent dibromobimane (DB) we show that a new fluorescent peak appears between M r 50 000 and 60 000. ATP—Pi exchange is very much decreased by DB. The results suggest that for regulation of ATP‐synthetase activity sulfhydryl groups in the region of the M r 31 000 protein(s) play an important role.Keywords
This publication has 16 references indexed in Scilit:
- Vesicular preparation of a highly coupled ATPase-ATP synthase complex from pig heart mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1982
- Crosslinking studies in oligomycin-sensitive ATPase from beef heart mitochondriaFEBS Letters, 1981
- The uncoupler‐binding protein in the proton‐pumping ATPase from beef‐heart mitochondriaFEBS Letters, 1981
- Discrimination between the N‐ethylmaleimide mersalyl‐sensitive protein(s) and the nucleotide translocator in pig heart mitochondriaFEBS Letters, 1980
- Is there a common binding center in the ADP, ATP carrier for substrate and inhibitors?FEBS Letters, 1980
- Identification of the N-ethylmaleimide reactive protein of the mitochondrial phosphate transporterBiochemistry, 1979
- Oligomycin‐sensitive ATPase from beef heart mitochondria: reaction with 2‐mercaptopropionylglycineFEBS Letters, 1978
- Free -SH variations during ATP synthesis by oxidative phosphorylation in heart muscle mitochondriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1971
- Implication of SH‐groups in the mitochondrial energy‐coupling system revealed by measurements of 14C‐ethacrynate incorporation into rat liver mitochondriaFEBS Letters, 1971
- Oligomycin and ADP sensitivity ofin vitroalkylation of SH-groups in rat liver mitochondriaFEBS Letters, 1970