Immunologic Studies of Hemoglobins

Abstract

1. Antisera to hemoglobin solutions obtained from normal adult, cord blood and sickle cell anemia erythrocytes have been prepared by immunizing rabbits with adjuvant mixtures. Furthermore, anti F hemoglobin sera have been similarly prepared by using purified fetal hemoglobin. A species specific anti guinea pig hemoglobin serum was also developed. 2. Although anti F hemoglobin sera appear to be specific for fetal hemoglobin, no such immunologic specificity could be detected between anti A and anti SCA sera obtained from rabbits. 3. It is suggested that sera prepared by using hemoglobin solutions obtained from normal and sickle cell anemia erythrocytes may contain anti F antibodies in addition to anti A and anti S precipitins and that fetal hemoglobin may be present in many human adult blood specimens.