THE THYROID STIMULATING HORMONE OF THE ANTERIOR PITUITARY AS DISTINCT FROM THE EXOPHTHALMOS PRODUCING SUBSTANCE1

Abstract
It was found in a variety of pituitary extracts studied that the thyroid stimulating hormone (TSH) and the exophthalmos producing substance (EPS) in anterior pituitary extracts do not always parallel one another. An analysis of the various chemical procedures by which these prepns. were obtained leads to the separation of TSH from EPS. These 2 substances were separated by their differential solubility in trichloracetic acid. EPS was assayed in Fundulus and TSH was assayed in day-old chicks. The results indicate that EPS and TSH are not the same substance.