The in Vitro Production of Erythropoietin and Thrombopoietin

Abstract

To determine any relationships between thrombopoietin, erythropoietin and erythrogenin supernatant fluids from culture lines of fetal mouse liver, fetal and adult bovine kidney and adult rabbit kidney cells were assayed for their content of the presumptive humoral regulators of hematopoiesis. Hormone production varied markedly from 1 culture line to another confirming other studies that the optimal culture conditions for the generation of hematologically-active hormones were not delineated. The lack of any discernable relationship between the production of thrombopoietin and erythropoietin, thrombopoietin and erythrogenin or erythropoietin and erythrogenin suggested that these materials represent 3 distinct entities.