Tissue Culture ofCinchona pubescens: Effects of Media Modifications on the Growth

Abstract

Studies were performed on the growth of callus cultures of Cinchona pubescens VAHL concerning the influence of the concentration of major and minor elements described by MURASHIGE and SKOOG [1], as well as a number of organic constituents in the medium. After 13 weeks a maximum increase of the fresh and dry weight was observed in tissues grown on a medium containing 2,4-D[ 1 ] (1 mg/l) and BA[ 1 ] (2 mg/l) as growth factors and with the same or the half concentration of mineral salts as used by MURASHIGE and SKOOG. 1 2,4-D: 2,4-Dichlorophenoxyacetic acid, BA: Benzyladenine