RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.
- 1 January 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (1), 160-164
- https://doi.org/10.1073/pnas.76.1.160
Abstract
The RNA produced in vitro by incubating nuclei from [human cervical carcinoma] HeLa cells infected with adenovirus serotype 2 was analyzed. Adenovirus-specific RNA is produced at a linear rate for up to 2.5 h. Hybridization analysis of RNA produced in nuclei isolated 18 h after infection indicated that transcription begins at the late promoter at map position 16.5. Sequence analysis of the 5''termini of the in vitro transcripts showed that this system initiates RNA chains de novo at the correct promoter and that the 5''terminus is capped.This publication has 23 references indexed in Scilit:
- Analysis of rna initiated in isolated mouse myeloma nuclei using purine nucleoside 5′[γ-S]triphosphates as affinity probesCell, 1978
- Two adenovirus mRNAs have a common 5′ terminal leader sequence encoded at least 10 kb upstream from their main coding regionsCell, 1977
- Spliced segments at the 5′ terminus of adenovirus 2 late mRNAProceedings of the National Academy of Sciences, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping by effects of ultraviolet irradiationCell, 1977
- Synthesis of messenger RNA-like molecules in isolated myeloma nucleiNucleic Acids Research, 1977
- Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamideNucleic Acids Research, 1977
- Transcription of ribonucleic acid in isolated mouse myeloma nucleiBiochemistry, 1973
- RNA metabolism in the HeLa cell nucleusJournal of Molecular Biology, 1966
- The ribonuclease activity of crystallized pancreatic deoxyribonucleaseAnalytical Biochemistry, 1966
- Biochemical studies on adenovirus multiplication. IX. Chemical and base composition analysis of 28 human adenoviruses.Proceedings of the National Academy of Sciences, 1965