Oligo- und Polynucleotidtrennungen, VI. Weitere Versuche zur Verteilungschromatographie

Abstract

Further experiments with partition chromatography on cellulose columns using a tri-n-butylamine containing solvent system are described. Serine specific t-RNAs have been partially purified on a preparative scale. Groups of oligonucleotides with the same chain length (tetra, hexa, and hepta respectively) can be further fractionated on these columns. In the same system the four mononucleotides are separated from each other, while oligoadenylic acids of different chain length are not. This indicates that also in poly- and oligonucleotide separations the nucleotide composition plays a bigger role than chain length.