CYCLOSPORIN RECEPTOR ON MOUSE LYMPHOCYTES

Abstract

Specific binding of [3H]-cyclosporin C (3H-CS-C), an immunosuppressive oligopeptide, was characterized on different mouse lymphocytes. The binding was saturable, time-dependent and reversible; a KO of 1.5 .times. 10-7 M and maximum binding capacity Bmax of 35 pmol/4 .times. 106 cells was found for thymocytes. A computerized analysis of the data confirmed a single population of high affinity binding sites. Lymphocytes of different organs differed in their Bmax:thymus > spleen > mesenteric lymph node (pure T cells) > mesenteric lymph node (pure B cells). Erythrocytes did not show a specific binding.